Journal: BMC Biotechnology

Article Title: pMAA-Red: a new pPZP-derived vector for fast visual screening of transgenic Arabidopsis plants at the seed stage

doi: 10.1186/1472-6750-12-37

Figure Lengend Snippet: pMAA-Red seed selection. Fluorescent seeds from transgenic Arabidopsis lines transformed with pMAA-Red ( A ) Selection of transformed seed (T 0 ) under microscope, ( B ) Silique in T 1 generation shows some non-florescent seeds (arrows) representing heterozygous line, ( C ) Silique in T 2 generation shows all florescent seeds representing homozygous line, ( D ) The selected homozygous line in T 2 generation.

Article Snippet: Seeds of transformed plants were harvested and photographed under an inverse microscope equipped with a DsRed fluorescence filter (Axiovert 200 M; Zeiss, Hallerbergmoos, Germany) and an integrated camera (AxioCam MRc5; Zeiss).

Techniques: Selection, Transgenic Assay, Transformation Assay, Microscopy

Journal: BMC Genomics

Article Title: Divergence of RNA localization between rat and mouse neurons reveals the potential for rapid brain evolution

doi: 10.1186/1471-2164-15-883

Figure Lengend Snippet: In situ hybridization reveals species-specific patterns of localization in neuronal dendrites. Fluorescent Microscopy evaluation of biotin-conjugated oligoprobes on paraformaldehyde fixed 14-day cultured rat and mouse cortical neurons hybridized with nine biotin-conjugated oligoprobes detected with streptadivin-Alexa Fluor 568 (Invitrogen). For each probe images set, the small bottom left corner panels represent MAP2 immuno-staining. Scale bar = 20 μm. (A) , Probes against SFRS16, ARHGDIA and HNRPK transcripts show higher dendritic localization in mouse neurons than in rat neurons (Red box). (B) , Probes against ZFP410, COMMD3 and RSP6 transcripts show higher dendritic localization in rat neurons than in mouse neurons (Blue box). (C) , Probes against UBA52, OLFM1 and H2AFZ transcripts show high dendritic localization in both rat and mouse neurons (Black box).

Article Snippet: The samples were visualized by fluorescent microscopy (Axiovert 200 M Inverted Fluorescent Microscope – Zeiss Inc., 20x Objective).

Techniques: In Situ Hybridization, Microscopy, Cell Culture, Immunostaining

Journal: BMC Genomics

Article Title: Divergence of RNA localization between rat and mouse neurons reveals the potential for rapid brain evolution

doi: 10.1186/1471-2164-15-883

Figure Lengend Snippet: Immunocytochemistry of protein localization in neuronal dendrites. Fluorescent microscopy evaluation of dendritically localized candidate proteins on cultured rat and mouse cortical neurons hybridized with nine primary antibodies against the protein of interest and detected with Alexa Fluor 546. For each primary antibody images set, the small bottom left corner panels represent MAP2 immuno-staining. Scale bar = 20 μm. (A) , Antibodies against SFRS16, ARHGDIA and HNRPK proteins show qualitatively higher dendritic localization in mouse neurons than in rat neurons (Red box). (B) , Antibodies against ZFP410, COMMD3 and RSP6 proteins show qualitatively higher dendritic localization in rat neurons than in mouse neurons (Blue box). (C) , Antibodies against UBA52, OLFM1 and H2AFZ proteins show dendritic localization in both rat and mouse neurons (Black box).

Article Snippet: The samples were visualized by fluorescent microscopy (Axiovert 200 M Inverted Fluorescent Microscope – Zeiss Inc., 20x Objective).

Techniques: Immunocytochemistry, Microscopy, Cell Culture, Immunostaining